Crohn's linked to gut protein

New Topic Post Reply Printable Version
[ << Previous Thread | Next Thread >> ]

Reef08
Regular Member


Date Joined Dec 2005
Total Posts : 267
   Posted 5/19/2008 10:45 AM (GMT -7)   
http://www.ansa.it/site/notizie/awnplus/english/news/2008-05-16_116222895.html

Sounds like leaky gut allows bacteria and toxins to infiltrate the gut and cause inflammation.

pb4
Elite Member


Date Joined Feb 2004
Total Posts : 20576
   Posted 5/19/2008 11:47 AM (GMT -7)   
I've heard of this a while back.

:)
My bum is broken....there's a big crack down the middle of it!  LOL  :)


Reef08
Regular Member


Date Joined Dec 2005
Total Posts : 267
   Posted 5/19/2008 11:54 AM (GMT -7)   
This is a new discovery though.

Mike W
Regular Member


Date Joined Aug 2006
Total Posts : 89
   Posted 5/19/2008 12:45 PM (GMT -7)   
But what causes the 'leaky gut' in the first place?
Diagnosed in 1999.
No surgeries.

Age : 31

Currently on 75mg 6mp , 20mg prednisone and probiotics.


EMom
Veteran Member


Date Joined Aug 2007
Total Posts : 990
   Posted 5/19/2008 12:59 PM (GMT -7)   
the *lack* of the protein. think of the protein as a protective barrier...no protein, bad guys come in and set up residence. thanks for the post, Reef!
EMom
Mother to 16 year old boy diagnosed in June, 2007.
Currently taking Asacol, omega 3s, digestive enzymes, probiotics, iron, vit. C, calcium w/D3 and a good multivitamin.
Started The Maker's Diet in early September. Gradually learning/using more SCD recipes, too! (cooking challenged)


Illini
Regular Member


Date Joined Dec 2007
Total Posts : 298
   Posted 5/19/2008 1:46 PM (GMT -7)   
A protein called lectin can also adhere to the gut and cause it to be "leaky." Lectins are found in legumes (beans). Incidentally beans are discouraged in the SCD, although it's due to the starch content.
July 2007 Drug-Induced Liver Injury
January 2008 Crohn's Ileitis
Currently trying...
Enteral Nutrition, Omega-3 (Flax Oil and Fish Oil), VSL#3, Vitamin E, Folic Acid, Psyllium


pb4
Elite Member


Date Joined Feb 2004
Total Posts : 20576
   Posted 5/19/2008 3:29 PM (GMT -7)   
Note the date...

Submitted 12 April 2007 ; accepted in final form 18 December 2007


ABSTRACT

Junctional adhesion molecule (JAM) is involved in tight junction (TJ) formation in epithelial cells. Three JAMs (A, B, and C) are expressed in rat hepatocytes, but only rat JAM-A is present in polarized WIF-B cells, a rat-human hepatic line. We used knockdown (KD) and overexpression in WIF-B cells to determine the role of JAM-A in the development of hepatic polarity. Expression of rat JAM-A short hairpin RNA resulted in 50% KD of JAM-A and substantial loss of hepatic polarity, as measured by the absence of apical cysts formed by adjacent cells and sealed by TJ belts. When inhibitory RNA-resistant human JAM-A (huWT) was expressed in KD cells, hepatic polarity was restored. In contrast, expression of JAM-A that either lacked its PDZ-binding motif (huC-term) or harbored a point mutation (T273A) did not complement, indicating that multiple sites within JAM-A's cytoplasmic tail are required for the development of hepatic polarity. Overexpression of huWT in normal WIF-B cells unexpectedly blocked WIF-B maturation to the hepatic phenotype, as did expression of three huJAM-A constructs with single point mutations in putative phosphorylation sites. In contrast, huC-term was without effect, and the T273A mutant only partially blocked maturation. Our results show that JAM-A is essential for the development of polarity in cultured hepatic cells via its possible phosphorylation and recruitment of relevant PDZ proteins and that hepatic polarity is achieved within a narrow range of JAM-A expression levels. Importantly, formation/maintenance of TJs and the apical domain in hepatic cells are linked, unlike simple epithelia.


partitioning-defective polarity protein/atypical protein kinase C complex

:)
My bum is broken....there's a big crack down the middle of it!  LOL  :)


map lady
Regular Member


Date Joined Feb 2008
Total Posts : 154
   Posted 5/19/2008 3:56 PM (GMT -7)   
Illini said...
A protein called lectin can also adhere to the gut and cause it to be "leaky." Lectins are found in legumes (beans). Incidentally beans are discouraged in the SCD, although it's due to the starch content.

Wait, so....if I had recently started eating beans every day in some form (protein + cheap + healthy) a few weeks before my intestines were suddenly like "HI WE'RE GOING TO GUSH BLOOD ALL DAY OK?".....I might have all these problems now due to bean chemicals attacking me? confused

Reef08
Regular Member


Date Joined Dec 2005
Total Posts : 267
   Posted 5/19/2008 3:59 PM (GMT -7)   
pb4 said...
Note the date...

Submitted 12 April 2007 ; accepted in final form 18 December 2007


ABSTRACT

Junctional adhesion molecule (JAM) is involved in tight junction (TJ) formation in epithelial cells. Three JAMs (A, B, and C) are expressed in rat hepatocytes, but only rat JAM-A is present in polarized WIF-B cells, a rat-human hepatic line. We used knockdown (KD) and overexpression in WIF-B cells to determine the role of JAM-A in the development of hepatic polarity. Expression of rat JAM-A short hairpin RNA resulted in 50% KD of JAM-A and substantial loss of hepatic polarity, as measured by the absence of apical cysts formed by adjacent cells and sealed by TJ belts. When inhibitory RNA-resistant human JAM-A (huWT) was expressed in KD cells, hepatic polarity was restored. In contrast, expression of JAM-A that either lacked its PDZ-binding motif (huC-term) or harbored a point mutation (T273A) did not complement, indicating that multiple sites within JAM-A's cytoplasmic tail are required for the development of hepatic polarity. Overexpression of huWT in normal WIF-B cells unexpectedly blocked WIF-B maturation to the hepatic phenotype, as did expression of three huJAM-A constructs with single point mutations in putative phosphorylation sites. In contrast, huC-term was without effect, and the T273A mutant only partially blocked maturation. Our results show that JAM-A is essential for the development of polarity in cultured hepatic cells via its possible phosphorylation and recruitment of relevant PDZ proteins and that hepatic polarity is achieved within a narrow range of JAM-A expression levels. Importantly, formation/maintenance of TJs and the apical domain in hepatic cells are linked, unlike simple epithelia.


partitioning-defective polarity protein/atypical protein kinase C complex

:)


Sorry, but the date was from 2 days ago. If you read carefully, the abstract you posted showed the protein in rats. Also, the abstract doesn't talk about IBD at all. It's about hepatic cells, which are totally unrelated. The latest article has found it could possibly cause it in humans. I'll make sure I check with you before I post anymore articles. eyes

Post Edited (Reef08) : 5/19/2008 5:06:41 PM (GMT-6)


Illini
Regular Member


Date Joined Dec 2007
Total Posts : 298
   Posted 5/19/2008 4:24 PM (GMT -7)   
Can't say for certain, map lady... but it's possible. It's not fully understood yet, but lectins appear to be bad news. They are called "antinutritionals" in the scientific literature! They bind to sugars on the cells lining the gut and actually go through into the bloodstream, where they cause red blood cells to clump together (possibly leading to anemia).

http://www.bmj.com/cgi/content/full/318/7190/1023#B14

Here's a quote from the article: "Among the effects observed in the small intestine of lectin fed rodents is stripping away of the mucous coat to expose naked mucosa and overgrowth of the mucosa by abnormal bacteria and protozoa." The rodents were fed kidney beans.


July 2007 Drug-Induced Liver Injury
January 2008 Crohn's Ileitis
Currently trying...
Enteral Nutrition, Omega-3 (Flax Oil and Fish Oil), VSL#3, Vitamin E, Folic Acid, Psyllium


ivy6
Elite Member


Date Joined Sep 2005
Total Posts : 10404
   Posted 5/19/2008 4:59 PM (GMT -7)   
Reef... pb..... be nice to each other, please. Remember, this is a support forum. How about we focus on the information in the article, and not when it was published. Even if it is oldish news, it will be new to many of our members.

I.
Co-Moderator Crohn's Forum.


broomhilda
Veteran Member


Date Joined May 2007
Total Posts : 1488
   Posted 5/19/2008 7:38 PM (GMT -7)   
yeah  Thanks PB4...it was "new" news to me!  However, it has put quite the damper on the chimichangas I was going to have for dinner tomorrow night!  Guess I'll have to stick to the avacodos and "fake mexican rice recipe". Dang it!  Thanks for keeping the underworld up to date!
New Topic Post Reply Printable Version
Forum Information
Currently it is Saturday, December 10, 2016 11:27 AM (GMT -7)
There are a total of 2,735,952 posts in 301,340 threads.
View Active Threads


Who's Online
This forum has 151440 registered members. Please welcome our newest member, Ian88.
264 Guest(s), 17 Registered Member(s) are currently online.  Details
scottishlymielady, PeteZa, blueberrymuffin, Girlie, Mad Martha, ChickNorris, Lynnwood, pmm73, PA_grandma, Ariel Smith, Traveler, bdavis, Tim Tam, iamamess, Tall Allen, Kristvet86, julymorning


Follow HealingWell.com on Facebook  Follow HealingWell.com on Twitter  Follow HealingWell.com on Pinterest
Advertisement
Advertisement

©1996-2016 HealingWell.com LLC  All rights reserved.

Advertise | Privacy Policy & Disclaimer