By taking human fetal intestinal cells and injecting them into mice and then injecting the mice with MAP and finding MAP to have been able to invade those cells, the researchers were able to (at least tentatively) debunk the long standing 'belief'/'argument' often held/given by MAP naysayers. Does this prove MAP can infect humans? Well MAP is found in humans, and this shows that it can infect human cells that were not inflamed prior to infection.
J Infect Dis. 2009 Feb 1;199(3):350-354.Click here to read Links
Mycobacterium avium paratuberculosis Invades Human Small-Intestinal Goblet Cells and Elicits Inflammation.
Golan L, Livneh-Kol A, Gonen E, Yagel S, Rosenshine I, Shpigel NY.
1The Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, Rehovot, and 2Department of Obstetrics and Gynecology, Hadassah University Hospital, and 3Molecular Genetics and Biotechnology, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
Crohn disease is a chronic inflammatory bowel disease of unknown etiology. Mycobacterium avium paratuberculosis (MAP) was found in the gut of patients with Crohn disease, but causality was not established. Fully developed, germ-free human small intestine and colon were established by subcutaneous transplantation of fetal gut into SCID (severe combined immunodeficiency) mice thereafter infected by direct intraluminal inoculation of MAP. We have found that MAP actively invades the human gut epithelial goblet cells of the small intestine, inducing severe tissue damage and inflammation. These observations indicate that MAP can specifically colonize the normal human small intestine and can elicit inflammation and severe mucosal damage.
PMID: 19133807 [PubMed - as supplied by publisher]