New Topic Post Reply Printable Version
[ << Previous Thread | Next Thread >> ]

poohbear37
Regular Member


Date Joined Feb 2007
Total Posts : 98
   Posted 11/5/2007 11:48 AM (GMT -7)   
My doctor said i have a positive ds DNA test.  He said the number was 21.5  can anyone put that into perspective for me?  Thanks!
~*~Kristyn~*~
Age - 24
Diagnosed w/UC at age 18
Fibromyalgia & Chronic Fatigue at age 16
Acid Reflux & Drug Induced Lupus 10/07 (from Remicade)
Current Meds - Asacol (12 pills daily)
-Nexium 40mg/day
-Canasa suppository (1000mg at bedtime)
-Lexapro (10mg)
-Prednisone (40mg when flaring)
-Multivitamin, Aloe Vera Gel


Ides
Forum Moderator


Date Joined Nov 2003
Total Posts : 7077
   Posted 11/5/2007 4:20 PM (GMT -7)   
That is the same antibody that I have from Remicade infusions. Do you know which test was used to measure your titer? There are 2-3 types of tests used to test for this and the vaules and ranges are different for each. That would help put the result in perspective.

My dsDNA titer usually is either 1:40 or 1:80 using the Crithidia method.

Here is an article that explains about the frequency of dsDNA antibodies in people that have had Remicade: Infliximab-Induced Autoantibodies in RA and AS


Moderator Crohn's Disease Forum
CD, Ankylosing Spondylitis, small fiber peripheral neuropathy, avascular necrosis, diffuse connective tissue disease, Sjogren's Syndrome ?
 


Gidget^
Regular Member


Date Joined Aug 2007
Total Posts : 171
   Posted 11/5/2007 5:14 PM (GMT -7)   

poohbear,
I've been doing a lot of reading on the Anti dsDNA because mine are so high.  I'd be glad to share with you the information I've come up with. 

I bet your lab tests are being done at either Quest Diagnostics or LabOne.  LabOne a few years ago came up with a new way to measure the antibodies.  Instead of the old method of diluting the blood till no antibodies could be found they've found a way to count each cell in a certain amount of blood if they had antibodies in them. A lot of labs are still reporting in the old method of titers.  The original ANA is now ANA-D or ANA-Direct.  It's a much more precise way of reporting how high the antibody levels are than in titers (i.e. (1:160, 1:320). 

At Quest Diagnostics or LabOne both the dsDNA and the ANA-D normal is 0-99, borderline is 100-120.  Anything above 120 is positive. 

According to the Lupus Foundation antibodies to DNA (ssDNA, dsDNA)(the protein that makes up the body's genetic code) are found primarily in SLE.  Disease activity correlates with a rise in Anti-DNA as well as other specific blood tests. 

From Hycor Biomedical the Anti-dsDNA antibodies have been suggested to be directly involved in the disease process, being included in the ARA criteria for the diagnosis of the disease. In SLE patients, levels of anti-dsDNA antibodies can be used in the monitoring of disease activity, since rapid increases have been reported to precede exacerbation of the disease and falls in antibody levels often accompany drug therapy.  Anti-dsDNA antibodies are not detected in the sera of patients with drug induced lupus.

I could keep pasting information I've gathered but it basically says the same thing, just different wording.  When dsDNA is positive, it indicates how active the lupus is right now in your body and a greater chance of kidney involvement.  The higher the number the worse it is.

Hope this helps.

 


Gidget^
 
SLE 9/07, FMS '90's, Spinal Stenosis, Osteoarthritis, Sinus Tach, PVC's, Kidney Stones, RLS, Parotid Neurofibroma 8/04, Cervical Cancer 9/06
Clinoril, Toprol XL, Prozac, Zyrtec-D, Zocor, Vitamins B6 & B12, Folic Acid, Loratab ,Percoset, Toradol
 
There is no psychiatrist in the world like a puppy licking your face.
 
 


poohbear37
Regular Member


Date Joined Feb 2007
Total Posts : 98
   Posted 11/5/2007 5:26 PM (GMT -7)   
It sounds like there are definatly alot of ways to get results from this test. My doctor said I was positive 21.5, where 5 or below is negative. it does not sound similar to the way your results were reported...hmmm..it is a very interesting subject.

Thanks!
~*~Kristyn~*~
Age - 24
Diagnosed w/UC at age 18
Fibromyalgia & Chronic Fatigue at age 16
Acid Reflux & Drug Induced Lupus 10/07 (from Remicade)
Current Meds - Asacol (12 pills daily)
-Nexium 40mg/day
-Canasa suppository (1000mg at bedtime)
-Lexapro (10mg)
-Prednisone (40mg when flaring)
-Multivitamin, Aloe Vera Gel


Gidget^
Regular Member


Date Joined Aug 2007
Total Posts : 171
   Posted 11/5/2007 8:20 PM (GMT -7)   
poohbear,
Your test results sent me back to investigating how results are reported. This is what I found:
They showed two samples and what the titer levels were and then Observed and Expected Returns. The closest your 21.5 result is the last sample of #2 ... 1:1600 which would be pretty high. I learned something new today! The url that I saw this at was http://www.orgentec.com/user_images/73/436674312_604Se.pdf. This company is not in the USA so maybe that is why they are reported different. Are you in the US poohbear?

Sample Dilution Observed [U/ml] Expected [U/ml]
1 1:100 104.2
1 1:200 50.6 52.1
1 1:400 24.9 26.1
1 1:800 11.2 13.0
2 1:200 135.3
2 1:400 68.9 67.7
2 1:800 35.2 33.8
2 1:1600 18.2 16.9


Gidget^
 
SLE 9/07, FMS '90's, Spinal Stenosis, Osteoarthritis, Sinus Tach, PVC's, Kidney Stones, RLS, Parotid Neurofibroma 8/04, Cervical Cancer 9/06
Clinoril, Toprol XL, Prozac, Zyrtec-D, Zocor, Vitamins B6 & B12, Folic Acid, Loratab ,Percoset, Toradol
 
There is no psychiatrist in the world like a puppy licking your face.
 
 


poohbear37
Regular Member


Date Joined Feb 2007
Total Posts : 98
   Posted 11/6/2007 5:12 AM (GMT -7)   
Thanks Gidget for doing all that research. Yes, I am in the US..in MAss. I am waiting for my actual blood reports to be sent to me so it will be interesting to see if they are reported in more than one way and the doctor chose to use the 21.5. He also wrote a letter using those numbers that he knows i will be taking to Mass general hospital for a 2nd opinion
~*~Kristyn~*~
Age - 24
Diagnosed w/UC at age 18
Fibromyalgia & Chronic Fatigue at age 16
Acid Reflux & Drug Induced Lupus 10/07 (from Remicade)
Current Meds - Asacol (12 pills daily)
-Nexium 40mg/day
-Canasa suppository (1000mg at bedtime)
-Lexapro (10mg)
-Prednisone (40mg when flaring)
-Multivitamin, Aloe Vera Gel

New Topic Post Reply Printable Version
Forum Information
Currently it is Wednesday, December 07, 2016 1:20 PM (GMT -7)
There are a total of 2,734,211 posts in 301,194 threads.
View Active Threads


Who's Online
This forum has 151319 registered members. Please welcome our newest member, Geezer Jock.
353 Guest(s), 17 Registered Member(s) are currently online.  Details
Chask, DBwithUC, JayMot, Bololidat, Chanymom, KAR90, dismissed, Mpac1, mpost, biker90, Mad Martha, pmm73, ChickenArise, catti, kcsmith72, straydog, iPoop


Follow HealingWell.com on Facebook  Follow HealingWell.com on Twitter  Follow HealingWell.com on Pinterest
Advertisement
Advertisement

©1996-2016 HealingWell.com LLC  All rights reserved.

Advertise | Privacy Policy & Disclaimer