Please everyone no going to nuts on this one. (too nuts)
This thread may look like an argument to some, but it is really so people can see other opinions and question-answer some things that need addressed.
Please, you dont have to participate if you dont like it. You can choose not to read a thread or ignore it.
We had a real good conversation going on the last post.
Many members wanted to discuss more.
Post Edited (+Lyme) : 7/22/2010 9:30:44 PM (GMT-6)
'The bands at the 31 kDa and 34 kDa positions of immunoblots are
produced by outer surface proteins A and B, respectively (OspA and OspB).
Blot interpretation criteria that could
best discriminate Lyme disease patients from controls, therefore, did not include
scoring antibodies to Osps A or B. When bands at 31 or 34 kDa are observed,
they are virtually always in the context of a robust IgG response to a large
number of scored antigens.
Why is OspA in particular is not scored
when it was the basis for an effective vaccine. People naturally think of the usual
way that vaccines work, neutralizing infection in a mammalian host, and expect a
vaccine antigen to be a good diagnostic antigen. They are unaware that the
OspA vaccine works by killing
OspA is well-expressed by
for antibodies that enter a tick during a blood meal from an OspA-vaccinated
host. When ticks are exposed to a blood meal and the body temperature of a
protein, OspC, is expressed instead . Reciprocal expression of these two
Osps has been demonstrated at the level of single cells. It is not surprising,
therefore, that antibody responses to OspC are diagnostically useful in early
Lyme disease, but responses to OspA are lacking
Some claim that patients
should be judged seropositive based on finding immunoblot bands solely at the
31- or 34-kDa positions, even when their serum is negative by an ELISA that
uses whole cell antigens. However,
A and B abundantly and ELISAs made from cultured whole cells contain
these antigens. Thus, samples from patients who have antibodies to Osps A or
B will react in a whole cell lysate ELISA. When an ELISA is negative but an
immunoblot of the same sample is scored positive, it is probable that faint
immunoblot bands are being “over-read.” '
Post Edited (nasalady) : 7/23/2010 12:09:19 PM (GMT-6)
Post Edited (+Lyme) : 7/23/2010 11:25:47 PM (GMT-6)