Replacement for daptomycin found in Zhang's Borrelia eradication protocol

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mpost
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Date Joined Feb 2015
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   Posted 10/7/2017 9:01 AM (GMT -6)   
New study from Zhang's team, highlighting again the fact that some essential oils are better than the most potent antibiotics.

Zhang will probably soon publish a combination of antibiotics and essential oils that will efficiently eradicate borrelia and can be tested in vivo. Please observe he found a replacement for Daptomycin (a very expensive drug, branded Cubicin in US, which also has poor BBB penetration, but was essential in complete eradication of Borrelia and biofilms in his tests).

Older daptomycin study here /www.ncbi.nlm.nih.gov/pubmed/25806811

It now can be replaced with Carvacrol a compound from Oregano oil, which crosses the BBB and has better activity against Borrelia persisters than daptomycin itself (!)

We already know cefoperazone (another weird antibiotic) can be replaced with a much more common cefuroxime
/www.ncbi.nlm.nih.gov/pmc/articles/PMC4748043/

So this is how a curative borrelia treatment looks like now:

- Minocycline/doxy
- Carvacrol (from oregano oil)
- Cefuroxime (brand name Ceftin)

The trick is to get the Carvacrol, i would not trust just taking oregano oil directly as the concentration of carvacrol will vary by a lot based on the brand, manufacturer, process, etc... So that is something that has to be achieved, extraction of high purity carvacrol from oregano oil and make it available as drug. Also note carcacrol alone will like not cure you, as it has to be used with Ceftin and Mino/doxy to target all borrelia forms.

The advantage of Carvacrol is it crosses the BBB, unlike daptomycin, so you can target the brain borrelia quite well now because all the 3 compounds above cross the BBB
/www.ncbi.nlm.nih.gov/pmc/articles/PMC3306416/

The newly published study about essential oils and borrelia biofilms/persisters can be found below:

Selective Essential Oils from Spice or Culinary Herbs Have High Activity against Stationary Phase and Biofilm Borrelia burgdorferi

Jie Feng1, Shuo Zhang1, Wanliang Shi1, Nevena Zubcevik2, Judith Miklossy3 and Ying Zhang1*
1Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, United States
2Department of Physical Medicine and Rehabilitation, Harvard Medical School, Spaulding Rehabilitation Hospital, United States
3International Alzheimer Research Centre, Prevention Alzheimer International Foundation, Switzerland

"We found that not all essential oils had activity against the B. burgdorferi stationary phase culture, with top 5 essential oils (oregano, cinnamon bark, clove bud, citronella, and wintergreen) at a low concentration of 0.25% showing high anti-persister activity that is more active than the known persister drug daptomycin.

Interestingly, some highly active essential oils were found to have excellent anti-biofilm ability as shown by their ability to dissolve the aggregated biofilm-like structures. The top 3 hits, oregano, cinnamon bark and clove bud completely eradicated all viable cells without any regrowth in subculture in fresh medium, whereas but not citronella and wintergreen did not have this effect. Carvacrol was found to be the most active ingredient of oregano oil showing excellent activity against B. burgdorferi stationary phase cells, while other ingredients of oregano oil p-cymene and α-terpinene had no apparent activity. "

/www.frontiersin.org/articles/10.3389/fmed.2017.00169/abstract

Sheeks175
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Date Joined May 2016
Total Posts : 155
   Posted 10/7/2017 2:45 PM (GMT -6)   
Thanks for posting! Super interesting information. I can't wait to see the study. If this is true, it would be so much more cost effective than daptomycin. It can be quite expensive.

Psilociraptor
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Date Joined Jul 2016
Total Posts : 997
   Posted 10/7/2017 4:24 PM (GMT -6)   
Cool beans. Probably not going to wait for someone to extract it. I'll get my protocol worked up and if i'm not seeing results I'll give this a shot and report back. I never thought it was systemic, but guess I might be wrong. Happy to be wrong in this case

Rikky1
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Date Joined Jun 2015
Total Posts : 2414
   Posted 10/7/2017 6:52 PM (GMT -6)   
i think its essential that anyone trying to eradicate these infections should look at various methods to eradicate. if you are taking a few abx then also add in a few herbs to work synergistically against the pathogens.

in terms of what to aid in assisting getting your life back i'm a HUGE proponent of DMSO, black cumin seed oil and oregano oil. though there are many other herbs and such that can help you these 3 are on the top of my list given their wide coverage and effectiveness.

blue skies
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Date Joined Jul 2017
Total Posts : 16
   Posted 10/8/2017 12:13 AM (GMT -6)   
It seems like this combination is the only one shown in research to "cure" lyme disease (although it's only in-vitro). Just wondering why it's not prescribed by all LLMDs if it's our best bet? I haven't taken this combination but would like to... although I also have babesia and bartonella but then at least lyme might be gone for good. If anyone's taken this combo it would be interesting to hear how it went for you. smile

mpost
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Date Joined Feb 2015
Total Posts : 1075
   Posted 10/8/2017 12:28 AM (GMT -6)   
take a look at the last link in my post. it says on top "Provisionally accepted The full-text will be published " - that paper is so new it has not been published yet....how do you expect LLMDs to have used it ? smile

and the rest of his team's findings are since 2016, that is very very recent when it comes to medical community.

also his initial paper last year had two antibiotics that are hard to find, very expensive and not good at BBB penetration: cefoperazone and dapto.

since then, Zhang's team has constantly refined their research, they got rid of one of them last year and replaced it with ceftin. now it seems carvacrol can replace daptomycin.

so a new treatment option seems to be emerging soon, to be tested on mice and to be tried by bold LLMDs like dr H from NY....

and Kim Lewis' team has to publish something soon too, they try slightly other drug types, more like new drug discovery.

stay put and do not lose hope....

k07
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Date Joined Sep 2015
Total Posts : 2039
   Posted 10/8/2017 6:38 AM (GMT -6)   
I was just thinking the other day that research news has been stagnant. Good to see this. When I first had symptoms in 2015 research was coming out like crazy and I though for sure in 2-3 years they will have a good combo to cure this...

mpost
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Date Joined Feb 2015
Total Posts : 1075
   Posted 10/9/2017 5:00 AM (GMT -6)   
we're getting there, but it's gonna take while.

All relevant antibiotic discovery research is funded by : GLA, Bay Area Lyme, and similar foundations.

The state funded research deals with some empiric stuff related to immune changes and genetic research but nothing more. So that's why is so slow... Even so, GLA has doubled its budget since 2014, after the merger, so that helps a lot, they have around $5m a year now.

mpost
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Date Joined Feb 2015
Total Posts : 1075
   Posted 10/11/2017 10:01 PM (GMT -6)   
full paper now available. the Discussion section is fascinating. Apparently carvacrol is a wonder drug, better than anything that was discovered so far, and crossing the bbb.

/doi.org/10.3389/fmed.2017.00169

i think the problem is taking the right amount of oregano oil and in the proper concentration.

sebreg
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Date Joined Jun 2015
Total Posts : 773
   Posted 10/11/2017 11:37 PM (GMT -6)   
Fantastic I'm going to take a close look at this tomorrow. Ironically enough I haven't tried oregano oil even though I've pretty much tried everything else under the sun. I'll have to try it soon. Another herb I'm finding very effective for my case is Olive Leaf Extract.

mpost
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Date Joined Feb 2015
Total Posts : 1075
   Posted 10/12/2017 5:56 AM (GMT -6)   
sebreg, if u want to see what this oregano oil can do to someone that has a very high borrelia load, watch this

/www.youtube.com/watch?v=-f5YA-zR7_Q

now the problem is oregano oil is not produced by a pharmaceutical, like Mino or Doxy are, it's made by a plant. So that means the
- potency of the extract will be different
- some vendors will plainly lie about the contents - like 90% cavacrol, give me a break, who's gonna test that and even if they do, the penalty is just a fine...
- there are dozens of oregano plant varieties with varied amounts of carvacrol...

what i plan to do is buy them from the same producer that Zhang used. in the study i pasted, go to Materials and Methods and check the text there

"Essential Oils and Drugs
A panel of commercially available essential oils was purchased from Plant Therapy (ID, USA), Natural Acres (MO, USA), or Plant Guru (NJ, USA). Carvacrol, p-cymene, and α-terpinene were purchased from Sigma-Aldrich (USA). Essential oils were added to BSK-H medium or B. burgdorferi cultures to form aqueous emulsion suspensions by vigorous vortexing, followed immediately by serially diluting the essential oil suspensions to desired concentrations into B. burgdorferi cultures. Essential oils were also dissolved in organic solvent dimethyl sulfoxide (DMSO) at 20%, followed by dilution at 1:20 into 7-day-old stationary phase culture to 1% final concentration. To make further dilutions for evaluating anti-Borrelia activity, the 1% essential oils were further diluted with the stationary phase culture to achieve desired dilutions. Dox, CefU (Sigma-Aldrich, USA), and (Dap) (AK Scientific, Inc., USA) were dissolved in suitable solvents (23, 24) to form 5 mg/ml stock solutions. The antibiotic stocks were filter-sterilized by 0.2 µm filter and stored at −20°C.
"

So... Plant Therapy (ID, USA), Natural Acres (MO, USA), or Plant Guru (NJ, USA).

k07
Veteran Member


Date Joined Sep 2015
Total Posts : 2039
   Posted 10/12/2017 7:17 AM (GMT -6)   
Let us know how it goes mpost and your dosage. I'm going to start with gaia brand. I have tried a lot of things too but never oregano oil. Are you going to be on abx too?

sebreg
Veteran Member


Date Joined Jun 2015
Total Posts : 773
   Posted 10/12/2017 6:01 PM (GMT -6)   
mpost said...
sebreg, if u want to see what this oregano oil can do to someone that has a very high borrelia load, watch this

/www.youtube.com/watch?v=-f5YA-zR7_Q

now the problem is oregano oil is not produced by a pharmaceutical, like Mino or Doxy are, it's made by a plant. So that means the
- potency of the extract will be different
- some vendors will plainly lie about the contents - like 90% cavacrol, give me a break, who's gonna test that and even if they do, the penalty is just a fine...
- there are dozens of oregano plant varieties with varied amounts of carvacrol...

what i plan to do is buy them from the same producer that Zhang used. in the study i pasted, go to Materials and Methods and check the text there

"Essential Oils and Drugs
A panel of commercially available essential oils was purchased from Plant Therapy (ID, USA), Natural Acres (MO, USA), or Plant Guru (NJ, USA). Carvacrol, p-cymene, and α-terpinene were purchased from Sigma-Aldrich (USA). Essential oils were added to BSK-H medium or B. burgdorferi cultures to form aqueous emulsion suspensions by vigorous vortexing, followed immediately by serially diluting the essential oil suspensions to desired concentrations into B. burgdorferi cultures. Essential oils were also dissolved in organic solvent dimethyl sulfoxide (DMSO) at 20%, followed by dilution at 1:20 into 7-day-old stationary phase culture to 1% final concentration. To make further dilutions for evaluating anti-Borrelia activity, the 1% essential oils were further diluted with the stationary phase culture to achieve desired dilutions. Dox, CefU (Sigma-Aldrich, USA), and (Dap) (AK Scientific, Inc., USA) were dissolved in suitable solvents (23, 24) to form 5 mg/ml stock solutions. The antibiotic stocks were filter-sterilized by 0.2 µm filter and stored at −20°C.
"

So... Plant Therapy (ID, USA), Natural Acres (MO, USA), or Plant Guru (NJ, USA).


Awesome, thank you for the info that is very helpful. Please let us know how it goes! The other thread on the oregano oil mentioned liposomal oregano oil by hopkinton pharmacy, this sounds interesting as well.

And K07, please let us know how your experience goes as well!

Lapis_29
Regular Member


Date Joined Sep 2017
Total Posts : 287
   Posted 10/21/2017 10:40 AM (GMT -6)   
thanks, I did not see this thread when posted

good info
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