I also have a speckled pattern pretty consistently - sometimes two patterns are reported - usually the other is nucleolar. Occasionally homogenous. I started out at 1:160 about
3 years ago. Lots of symptoms. When it reached 1:1280 I was started on Plaquenil - but still only have a dx of UCTD (undifferentiated connective tissue disease). Other blood markers for Lupus remain negative, with a mildly positive rheum. factor.
Since then, the ANA has been all over the place - down to 1:80, and even once I think it was negative. I saw a dermatologist a couple weeks ago. She suspected Sjogren's so did labs. The ANA came back at 1:320. One week later I had an appt. w/ my rheumy. I showed him the results (neg. for Sjogren's). He decided to run his own labs, since I was due for some others. He reminded me that the ANA was not an indicator for disease activity, but was just a marker. He ran another ANA anyway, however, and the result was 1:160. One week later. Different lab.
I have also heard that once a positive ANA has been established - the test doesn't really need to be repeated. But the other markers for Lupus, RA, MCTD etc. should be repeated from time to time as they can change. Rheumatologists seem to have individual ideas about this. At any rate, your symptoms are what's most important and need to be addressed. www.lupus.org is a good place to start for information.
In the meantime - keep track of your symptoms. It may take awhile to get to a diagnosis. Don't be afraid to return to your GP or rheumy if your symptoms increase. They should be sharing information.