Part 1: www.healingwell.com/community/default.aspx?f=30&m=3834079
Part 2: www.healingwell.com/community/default.aspx?f=30&m=3849152
Part 3: www.healingwell.com/community/default.aspx?f=30&m=3859907
To recap the conversation:
I use a E200 Nikon-grade microscope with a fluorescent attachment and acridine orange. I could barely see anything with a cheap microscope initially in brightfield. Most videos I have seen online that people do will never see all these forms. Regular brightfield will not work well and many spirochetes are inside the RBCs. Phase contrast provided incredible ability to learn about how these morph, which I did for about a year. I would go back and read the science info after I would notice various elements in phase contrast. This form is very common and you can find it all over online, not just in scientific journals, but even an generic Google images searches.
Acridine orange will kill these bacteria. They are dead, and they fluoresce with RNA especially when they are in their aggregation and building mode. When the L-bodies made up of spirochetes become more cohesive, the typically fluoresce green or yellow expressing DNA. It doesn't matter that they are dead, as the L-form scientist who I corresponded with told me as long as I could get a good micrograph. When you have caught many of the forms that Dr. Mattman and MacDonald described, and when you start to have way more known morphologies than you can find in scientific journals, than I can see what the L-form scientist meant. Borrelia L-forms are unique among L-forms, as my videos point out by quoting Dr. Mattman. Unlike other L-forms, they often have spirochetal forms protruding rather than infinite varieties of rhizoid forms.
Acridine orange has opened up a whole new world to me. I have never seen anyone with multi-spirochetal cysts, other than in scientific journals before I found 100's of them. This is not because of anything I did, but I believe that many of you would find exactly the same things. We are entering taboo-land now. Why if this is so easy and acridine orange has been around for decades, do we not have a scientific catologue with tens of thousands of images? They don't want us to realize that these are so easy to find. Yes, I grant that my family may have B. Miyamoti, B. Mayonii, additionally, but all borrelia is by definition relapsing fever bacteria due to antigenic variation. However, with some blood drops of my family with tens of thousands of microbes, I think multiple kinds of borrelia is probable.
I never stopped and asked - what makes up an L-form when I first started doing this. But obviously with the case of borrelia, spirochetes build them. The spirochetes themselves aggregate, inflate and form the L-body, or spirochetes work together to put a thin wall around other members of the aggregate.
I have made some videos about the Mattman long form L-body, that many mistake for a nematode. I thought they were nematodes too, but I had a scientist do DNA testing for nematodes and it was negative. Then many nematode scientists watched some video, and some admitted they look like nematodes, but they said that they are definitely not. I kept studying them with acridine orange, and many looked just like the smaller L-bodies, with spirochetal morphologies inside and protruding from them. This is exactly how Dr. Mattman described the borrelia L-body. Finally, I caught the preliminary stages of borrelia "Mattman long L-form" building and they were made up of spirochetes building a large L-body that was spirochetal shape. They do this all the time. I thought to myself - "all these years there is nothing about these from scientists" since Dr. Mattman pointed them out? They are the easiest diagnostic aid imaginable - how could they miss this target with borrelia antibodies or as an indicator for preliminary diagnostic screening that results in further testing? These Mattman long forms are taboo-land again. But they are in every single sample I test - many of them. Borrelia is arrogant and it likes to form a large shape like itself. I am going to post a short video with a couple of these Mattman long forms that were in the single drop of blood that you just watched a video of - one is loaded with spirochetal morphologies and one is building a cohesive long L-form, where part of it is spirochetes aggregating to build the corpus, and part of it is fluorescing green.
I do hope that many others will start to use acridine orange.
Here's a link to JohnB2's YouTube: /www.youtube.com/channel/UCMcCeO0tG4R4HOy8fFYIEnA
Yes, that is a spirochete that is drilling very fast in the plasma & around red blood cells of someone who is chronically ill for 40 years. Even in ticks some spiros can drill this fast.
John B, Acridine Orange can also stain indiscriminately & I had seen your AO stain in the past & those are not spirochetes. What happens is that the wbc's lyse & get strewn all over in the smear & you will get string-like subjects that are really part of the DNA from the Wbc's.For you to get a better idea, stain a normal persons blood & you will see the same artifacts. Full strength AO will be cytotoxic to Borrelia & can destroy most of them. Lab workers have also reported that full strength AO will also lyse sperm when they tried it & so they know it is toxic to certain cells in it's purer forms. Spirochetes are hardy yet delicate creatures.
I use live cell AO staining, in which I dilute AO .2% with NaCl (Salt water) & I give the exact dilution method that I used in the description of my video. Too concentrated & it can kill the spiros, not concentrated enough & one can miss the spiros if they are there. Sometimes even when you get the concentration right even the amount of AO drop that you use with the blood drop can alter the outcome, as it is hard to control the exact amount of a drop without a pipette.
When you look for AO stains in live preps, look for the known shapes, movements & morphologies (SOP or Tennis Rackets...etc) & not just any strings or string conglomerates. I hope this helps, as I want you to be successful too...all the best and if you need any help just LMK.
String of Pearls & 2 daughter splitting spiros in this video can be seen, as well as blebs/granular forms. Not all the green dots are spiros though, as some are from wbc components (lysosomes & peroxisomes).