One thing I don't like is the obfuscation of persister states.
yes Psilociraptor i too think that listing of terminology is not useful. Especially that it was proven borrelia does not form structures that are traditionally identified as "cysts". This has been shown beyond any doubt by Leona Gilbert's team from Jyväskylä university, Finland, in 2015:/www.ncbi.nlm.nih.gov/pmc/articles/PMC4339653/
...with some beautiful pictures done with the electron microscope, you can see the cysts are actually not cysts.
The author(s) write:
"Previously, the round bodies (RBs) of B. burgdorferi have been ambiguously named in various ways. These terms include CWD and L-forms, spheroplasts, protoplasts, propagules and even cysts (Domingue & Woody, 1997; Stricker & Johnson, 2011). Nonetheless, all of these labels describe the same spherical structures. This terminology is confusing and makes presumptions about
the biochemical and morphological characteristics of B. burgdorferi RBs, such as a lack of cell wall (CWD, spheroplasts and protoplasts), or that these forms are encysted with a capsulated outer membrane (cysts). However, the cell envelope components and morphology of B. burgdorferi RBs have not been clearly studied before.
Here we confirmed for the first time that RBs actually have an intact cell envelope with a peptidoglycan layer (Figs 5 and and7),7), indicating that they do not fulfil clearly the definitions of spheroplasts, CWD or encysted forms although there are some modifications in the cell envelope and cell wall architecture
. Furthermore, the intact cell envelope of RBs (Fig. 5), similar to the spirochaete, provides evidence for the previous suggestion (Alban et al., 2000) that RBs are not degrading cells. To avoid confusing terminology, we suggest that B. burgdorferi spherical shapes are termed ‘round bodies’ to describe these forms better.
So yes you are right, Borrelia DOES NOT MAKE CYSTS. Let's call these round and dormant things "persisters" or round bodies. Sorry for the caps
Okay - I have wondered if persisters and “cysts” were one and the same.
So please clarify for me - there are spirochetes and persisters. What about
biofilms? Are these clusters of spirochetes surrounded by a biofilm? What about
the round bodies - are they included in the biofilm colonies?
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Symp started April/2013; Buhner's Lyme May 15-July24/14; Igenex pos. July 3/14
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