Mold Sick Folks! There is a specific bacteria that can help you

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Lapis_29
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   Posted 4/12/2018 7:05 PM (GMT -6)   
there are now multiple studies which show that Lactobacillus reuteri binds to and detoxifies the mold toxin aflatoxin B1.

There are multiple products with this probiotic strain, I am using one from Nature's Way and it is definitely helping. There are other brands that I plan on trying.

If you try this please post here and let other know if it works for you, thanks

Somebody said...


Assessment of Aflatoxin B1 Binding to Lactobacillus reuteri by Microscopy and Fluorescence Techniques

Download citation https://doi.org/10.1080/08905436.2011.576561



Abstract


The aims of this research were to study the applicability of flow cytometry to detect aflatoxin bound to Lactobacillus reuteri based on fluorescence intensity patterns, and to evaluate the aflatoxin/bacteria interaction by microscopy techniques based on fluorescent staining and disruption of the cell wall structure. Our results demonstrated a specific interaction between the AFB1 bound to the cell surface and the FITC-labeled monoclonal antibody against the aflatoxin B1 used. Microscopy assays confirmed for first time in a clear visual way the aflatoxin-bacteria interaction, and that aflatoxin binding causes changes that alter the bacterial cell surface.

The methods applied in our study are suitable for detection of aflatoxin bound to the cell surface of Lactobacillus reuteri and possibly by other microorganisms, and proved to be a potential tool for research and clinical detection of human exposure to aflatoxins.


Lapis_29
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   Posted 4/12/2018 7:07 PM (GMT -6)   
Somebody said...


Effect of oral supplementation of Lactobacillus reuteri in reduction of intestinal absorption of aflatoxin B(1) in rats.

Abstract

The goals of this work were to assess the ability of Lactobacillus reuteri to bind aflatoxin B(1) in the intestinal tract and determine its effect on intestinal absorption of the toxin dispensed in either single or multiple doses in a murine model. Male Wistar rats were used, and two experiments were conducted after bacteria were implanted. Experiment one involved a single-oral dose of toxin, and the subsequent flow cytometric analysis of bacteria isolated from the small intestine and treated with specific FITC-labeled AFB(1) antibodies.

The second experiment was carried out supplying the toxin in 7 oral sub-doses, and the later quantification of AFB(1)-Lys adducts in blood samples by ELISA assay. The results demonstrated that L. reuteri was able to bind AFB(1) in the intestinal tract, mostly in the duodenum. Furthermore, the AFB(1)-Lys adducts were present at significantly lower levels in those animals receiving AFB(1) plus bacteria than in those receiving only AFB(1).

Our findings confirm that probiotic bacteria could act as biological barriers in normal intestinal conditions thereby reducing the bioavailability of AFB(1) ingested orally in a single or multiple doses, thus avoiding its toxic effects.


Lapis_29
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   Posted 4/12/2018 7:09 PM (GMT -6)   
Lactobacillus reuteri NRRL14171

that is the specific strain used in this first study

Lapis_29
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   Posted 4/12/2018 7:14 PM (GMT -6)   
Lactobacillus plantarum also binds to aflatoxin B1

Somebody said...


Reduction of Aflatoxin B1 Toxicity by Lactobacillus plantarum C88: A Potential Probiotic Strain Isolated from Chinese Traditional Fermented Food “Tofu”

Abstract

In this study, we investigated the potential of Lactobacillus plantarum isolated from Chinese traditional fermented foods to reduce the toxicity of aflatoxin B1 (AFB1), and its subsequent detoxification mechanism. Among all the investigated L. plantarum strains, L. plantarum C88 showed the strongest AFB1 binding capacity in vitro, and was orally administered to mice with liver oxidative damage induced by AFB1. In the therapy groups, the mice that received L. plantarum C88, especially heat-killed L. plantarum C88, after a single dose of AFB1 exposure, showed an increase in unabsorbed AFB1 in the feces.

Moreover, the effects of L. plantarum C88 on the enzymes and non-enzymes antioxidant abilities in serum and liver, histological alterations of liver were assayed. The results indicated that compared to the control group, L. plantarum C88 alone administration induced significant increase of antioxidant capacity, but did not induce any significant changes in the histological picture. Compared to the mice that received AFB1 only, L. plantarum C88 treatment could weaken oxidative stress by enhancing the activity of antioxidant enzymes and elevating the expression of Glutathione S-transferase (GST) A3 through Nuclear factor erythroid (derived factor 2) related factor 2 (Nrf2) pathway. Furthermore, cytochrome P450 (CYP 450) 1A2 and CYP 3A4 expression was inhibited by L. plantarum C88, and urinary aflatoxin B1-N7-guanine (AFB-N7-guanine), a AFB1 metabolite formed by CYP 1A2 and CYP 3A4, was significantly reduced by the presence of viable L. plantarum C88.

Meanwhile, the significant improvements were showed in histological pictures of the liver tissues in mice orally administered with viable L. plantarum C88. Collectively, L. plantarum C88 may alleviate AFB1 toxicity by increasing fecal AFB1 excretion, reversing deficits in antioxidant defense systems and regulating the metabolism of AFB1.


Lapis_29
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   Posted 4/12/2018 7:21 PM (GMT -6)   
Lactobacillus rhamnosus also binds to aflatoxin B1, most affective of the 12 bacterial strains tested

Somebody said...


Surface Binding of Aflatoxin B1 by Lactic Acid Bacteria

ABSTRACT

Specific lactic acid bacterial strains remove toxins from liquid media by physical binding. The stability of the aflatoxin B1 complexes formed with 12 bacterial strains in both viable and nonviable (heat- or acid-treated) forms was assessed by repetitive aqueous extraction. By the fifth extraction, up to 71% of the total aflatoxin B1 remained bound. Nonviable bacteria retained the highest amount of aflatoxin B1. Lactobacillus rhamnosus strain GG (ATCC 53103) and L. rhamnosus strain LC-705 (DSM 7061) removed aflatoxin B1 from solution most efficiently and were selected for further study.


Lapis_29
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   Posted 4/12/2018 7:30 PM (GMT -6)   
Bifidobacterium infantis also binds to and detoxifies the mold toxin aflatoxin B1, very strongly

Somebody said...


Strains of probiotic bacteria showed diverse levels
of detoxification properties to aflatoxin B1. Generally,
there were no significant differences in detoxification
ability of the strains and duration of exposure of the
bacterial cells with the toxin. B. pseudolongum 20099
demonstrated a detoxification ability of 50% of the
added aflatoxin B1 as compared to other strains during
the 180 min treatment period. This strain also showed
the least toxin binding strength among all the strains
studied.

B. infantis 1912 showed strong toxin binding
ability and strength. The detoxification properties of
most probiotic bacteria were dependent on the cell
amount.


The findings of this study suggest that some
strains of probiotic bacteria can be used for removal of
aflatoxins in contaminated foods.

Girlie
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Date Joined May 2014
Total Posts : 32695
   Posted 4/12/2018 7:38 PM (GMT -6)   
I see Lactobacillus in a lot of the probiotics.

does it need to be Rhamnosus, plantarum or reuteri?

I should check if mine just say lactobacillus or if it specifies which one.
Moderator, Lyme Forum
Symp started April/2013; Buhner's Lyme May 15-July24/14; Igenex pos. July 3/14
Doxy: July 4-Aug.24/14;Zithro July26-Aug24/14; Amox + Proben. Aug. 29/14;
added biaxin Sept. 26/14
Disc. amox,added Ceftin Nov. 20th.;
Disc. biaxin added Buhner bart herbs Dec/14;Jan/15 pulsing Tinda (w/ Ceftin);
Abx/herb break Apr-July/15; July-mino; Aug. added Rif;
Nov./15 mino - to biaxi

Lapis_29
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Date Joined Sep 2017
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   Posted 4/12/2018 7:45 PM (GMT -6)   
Girlie said...
I see Lactobacillus in a lot of the probiotics.

does it need to be Rhamnosus, plantarum or reuteri?

I should check if mine just say lactobacillus or if it specifies which one.


yes, needs to be those specific strains

Girlie
Forum Moderator


Date Joined May 2014
Total Posts : 32695
   Posted 4/12/2018 7:53 PM (GMT -6)   
I just checked mine:

80% Lactobacillus rhmanosus
10% Lactobacillus acidophilus
10% Bifidobacterium bifidum

That one was one i picked up from our health food store.


Another one I have doesn't have any of the 'good' stuff and it's the one recommended by my LLND

I'm out of my third one - so can't check it.
Moderator, Lyme Forum
Symp started April/2013; Buhner's Lyme May 15-July24/14; Igenex pos. July 3/14
Doxy: July 4-Aug.24/14;Zithro July26-Aug24/14; Amox + Proben. Aug. 29/14;
added biaxin Sept. 26/14
Disc. amox,added Ceftin Nov. 20th.;
Disc. biaxin added Buhner bart herbs Dec/14;Jan/15 pulsing Tinda (w/ Ceftin);
Abx/herb break Apr-July/15; July-mino; Aug. added Rif;
Nov./15 mino - to biaxi

Lapis_29
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Date Joined Sep 2017
Total Posts : 846
   Posted 4/12/2018 8:01 PM (GMT -6)   

The Dude Abides
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Date Joined May 2017
Total Posts : 1135
   Posted 4/12/2018 8:33 PM (GMT -6)   
Good information, Lapis_29. Thank you.

ChickenArise
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Date Joined Nov 2015
Total Posts : 1510
   Posted 4/13/2018 7:36 AM (GMT -6)   
Very resourceful as always. Thank you, Lapis.

2014 Mold Sick,2015 Clinical Lyme and co.,2016 Morgellons,2017 Remission
YT: ClintFromNYtoVA2
Twitter: @ClintFromNYtoVA
Blog: www.fascinatingtimetobealive.blogspot.com/
" The path to disappointment is paved with expectations "

Lapis_29
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Date Joined Sep 2017
Total Posts : 846
   Posted 4/13/2018 11:48 AM (GMT -6)   
sodium bentonite MUCH better aflatoxin binder than calcium bentonite, make sure you get the right one

activated carbon, had no effect on aflatoxin

Somebody said...


Aflatoxin binders II: reduction of aflatoxin M1 in milk by sequestering agents of cows consuming aflatoxin in feed.

Abstract

Sequestering agents bind dietary aflatoxin B1 (AFB1) and reduce absorption from an animal's gastrointestinal tract. As a result, they protect an animal from the toxic effects of AFB1 and reduce transfer of the metabolite, aflatoxin M1 (AFM1), into milk. Three experiments, using late-lactation Holstein cows fed AFB1-contaminated feed, were conducted to evaluate several potential sequestering agents for their abilities to prevent or reduce the transmission of AFM1 into milk.

Six agents previously tested in our laboratory for AFB1 binding in vitro were evaluated in these experiments. These were: SA-20, an activated carbon (AC-A); Astra-Ben-20, a sodium bentonite (AB-20); MTB-100, an esterified glucomannan (MTB-100); Red Crown, a calcium bentonite (RC); Flow Guard, a sodium bentonite (FG); and Mycrosorb, a sodium bentonite (MS). Five of the six sequestering agents significantly (P < 0.01) reduced AFM1 contamination of milk (AB-20, 61%; FG, 65%; MS, 50%; MTB-100, 59%; and RC, 31%); whereas, AC-A, activated carbon, had no effect on AFM1 transmission at 0.25% of feed.

By the first milking (1 day after cows consumed contaminated feed), AFM1 appeared in milk, then reached maximum levels after three days, and was absent from milk within four days after AFB1 was removed from the feed. Sodium bentonites at 1.2% of feed showed good potential as AFB1 binders; MTB-100, a yeast cell wall product, was equally effective at 0.05% in feed. Potential AFB1 binding agents should be evaluated experimentally to demonstrate efficacy. Our data show that sequestering agents can reduce AFM1 in milk of cows fed AFB1-contaminated feed.


Lapis_29
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Date Joined Sep 2017
Total Posts : 846
   Posted 4/13/2018 12:00 PM (GMT -6)   
chlorophyll binds to the mold toxin aflatoxin

you can get chlorophyll caps on amazon

Somebody said...


Effects of chlorophyll and chlorophyllin on low-dose aflatoxin B(1) pharmacokinetics in human volunteers.

Abstract

Chlorophyll (Chla) and chlorophyllin (CHL) were shown previously to reduce carcinogen bioavailability, biomarker damage, and tumorigenicity in trout and rats. These findings were partially extended to humans, where CHL reduced excretion of aflatoxin B(1) (AFB(1))-DNA repair products in Chinese unavoidably exposed to dietary AFB(1). However, neither AFB(1) pharmacokinetics nor Chla effects were examined. We conducted an unblinded crossover study to establish AFB(1) pharmacokinetic parameters among four human volunteers, and to explore possible effects of CHL or Chla cotreatment in three of those volunteers. For protocol 1, fasted subjects received an Institutional Review Board-approved dose of 14C-AFB(1) (30 ng, 5 nCi) by capsule with 100 mL water, followed by normal eating and drinking after 2 hours. Blood and cumulative urine samples were collected over 72 hours, and 14C- AFB(1) equivalents were determined by accelerator mass spectrometry. Protocols 2 and 3 were similar except capsules also contained 150 mg of purified Chla or CHL, respectively. Protocols were repeated thrice for each volunteer. The study revealed rapid human AFB(1) uptake (plasma k(a), 5.05 + or - 1.10 h(-1); T(max), 1.0 hour) and urinary elimination (95% complete by 24 hours) kinetics.

Chlorophyll (Chla) and chlorophyllin (CHL) treatment each significantly impeded AFB(1) absorption and reduced Cmax and AUCs (plasma and urine) in one or more subjects.

These initial results provide AFB(1) pharmacokinetic parameters previously unavailable for humans, and suggest that Chla or CHL co-consumption may limit the bioavailability of ingested aflatoxin in humans, as they do in animal models.


ChickenArise
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Date Joined Nov 2015
Total Posts : 1510
   Posted 4/13/2018 2:34 PM (GMT -6)   
Lapis_29 said...
sodium bentonite MUCH better aflatoxin binder than calcium bentonite, make sure you get the right one

activated carbon, had no effect on aflatoxin



However activated carbon (charcoal) does remove Fumosinin.

Fumonisin B1 is the most prevalent member of a family of toxins produced by several species of Fusarium molds, which occur mainly in different varieties of corn.

Fumonisin is well known to cause many insults to mammals. For example, induced cancers in lab animals have included: liver adenomas and carcinomas, skin cancer, kidney tubule carcinomas, Simply, Fumonisin B1 is highly toxic to both livers and kidneys. It kills cells. It induces DNA damage. It undermines key reactions in lab animals and humans: sphingolipid, phospholipid and fatty acid metabolism is disturbed in and outside the body in all animals and in a single human study. Disruption of sphingolipids by fumonisin B1 causes cell death.

Comparing the Mold Toxin Binders on Fumosinin:

Cholestyramine showed the best adsorption capacity. It was quite effective at 85% absorption.

Activated Carbon had the second best mold toxin absorption at 62%.

Bentonite clay adsorbed minimally. Even when the amount of toxin load in the water was reduced to a low load of only 13 microg/ml, the Bentonite clay still only bound 12% of the toxin.

This is why it is important to rotate the binders when you are not aware of which mycotoxins you were exposed to. They have different effectiveness for different mycotoxins.

2014 Mold Sick,2015 Clinical Lyme and co.,2016 Morgellons,2017 Remission
YT: ClintFromNYtoVA2
Twitter: @ClintFromNYtoVA
Blog: www.fascinatingtimetobealive.blogspot.com/
" The path to disappointment is paved with expectations "
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