Dark-Field Microscopy.....how to prepare? Stir up the bugs??

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Regular Member

Date Joined Apr 2015
Total Posts : 77
   Posted 4/21/2018 8:00 AM (GMT -6)   
Hi all,

I have an appointment for dark-field microscopy in just under 2 weeks. Given that they'll be looking at fresh live blood for spirochetes, or anything else interesting I suppose........is there anything I can do to "encourage" any pathogens to be in the blood, in a more detectable form?

I know there's a lot of talk about cyst forms, spirochetes, pluromorphs (sp??), etc., and how different treatments tend to drive bacteria in / out of different modes. But I guess my foggy brain hasn't wrapped itself around that stuff yet. And I'm not really familiar with DFM or what it can and cannot do.

FWIW, I have been on the Cowden protocol at a somewhat reduced level for a month or so, after a month off, after a few months on. Also have some of the other herbs available, and doxy, tinidazole and metronidazole.

So, any advice? smile


Post Edited (Lyme_CJ) : 4/21/2018 8:31:08 PM (GMT-6)

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Date Joined May 2014
Total Posts : 33837
   Posted 4/21/2018 1:47 PM (GMT -6)   
I know when people do the DNA Connexions testing they do some light exercise and/or massage before they test...although that's urine, and not blood...not sure if it would work.

Worth a try maybe?

We have several members who do the Microscopy - hopefully they will see this thread and comment.
Moderator, Lyme Forum
Symp started April/2013; Buhner's Lyme May 15-July24/14; Igenex pos. July 3/14
Doxy: July 4-Aug.24/14;Zithro July26-Aug24/14; Amox + Proben. Aug. 29/14;
added biaxin Sept. 26/14
Disc. amox,added Ceftin Nov. 20th.;
Disc. biaxin added Buhner bart herbs Dec/14;Jan/15 pulsing Tinda (w/ Ceftin);
Abx/herb break Apr-July/15; July-mino; Aug. added Rif;
Nov./15 mino - to biaxi

Veteran Member

Date Joined Nov 2015
Total Posts : 4895
   Posted 4/21/2018 9:12 PM (GMT -6)   
teasel supposedly lures em out ...have her crush red blood cells at end, to really see them. ask em to do a giemsa stain with brightfield for babs etc

Regular Member

Date Joined Apr 2015
Total Posts : 77
   Posted 4/21/2018 9:36 PM (GMT -6)   
Thanks for the responses!

bluelyme - on the teasel, is that something that I would take for, say, a few days or a week? That is kind of what I was thinking as well. I seem to remember that on the Igenex urine test that I did a while back, they mentioned a couple of different protocols that some specific drs. recommend. To lure them out, as you say.

I have more research to do.....

EDIT - I meant to mention, I did ask at the clinic I'll be going to, if there's anything I can do to prepare. Either there was a misunderstanding (I don't know if I specified preparing for the test vs. just an appointment, at least not well), or they didn't have anything particular to recommend. I'm hoping I don't get there and visit with the doc and he says I should've something something. Maybe I'm just worrying again!

Post Edited (Lyme_CJ) : 4/21/2018 8:41:11 PM (GMT-6)

Veteran Member

Date Joined Nov 2015
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   Posted 4/21/2018 11:12 PM (GMT -6)   
some say just dont eat a snack within a few hours , if they know how to crush or leave under lamp to degrade then all the bugs inside come out ...nice nasty spectacle...teasel may herx? i would use kineseology to get daily dosing...depends on strength of tincture and your constitution i reckon...

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Date Joined Feb 2015
Total Posts : 1527
   Posted 4/22/2018 1:16 AM (GMT -6)   
you can take serrapeptase before. it breaks biofilms and that should get more bacteria in the blood theoretically...

i know people say lyme is a tissue disease and if u have it for a long time u cannot find spirochetes in blood, but that's not true. after 5 years post infection and 3 months of aggressive antibiotic treatment back in 2015 i still had live spirochetes and bartonella in my blood. that's when i know things are seriously messed up with this disease... it's one to relapse months after antibiotic, and completely other to take a cocktail of antibiotics and 12h later to find live moving spirochetes and bart in your blood. They seemed very much unaffected by my treatment sad

So if you are good, you know where to look and find them. But if you are not trained lab pathologist you may easily confuse them (borrelia) with collagen filaments.

The real bacteria needs to have the form but also telltale movements, at least on my DFM bulletin said mine had both.

I know there are many people buying equipment to spot it at home but i think science is something that requires people to read and study before they become doctors or lab technicians. So it is good you go to a lab to have this checked and you are not playing "dr Goodhealth" in the basement...

Post Edited (mpost) : 4/22/2018 12:19:34 AM (GMT-6)

Veteran Member

Date Joined Nov 2015
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   Posted 4/22/2018 11:12 PM (GMT -6)   
the only thing to have a visable effect for me was bvt with herbs ....reg docs labs and pathologists left me to die ...dr "goodhealth saved this catscratch feverite...fibrin is easy to spot and distinguish imho

Regular Member

Date Joined Nov 2016
Total Posts : 158
   Posted 4/23/2018 8:17 PM (GMT -6)   
The best thing to do is maybe to stop the treatments 1 week before.
And to be fasted. If you are not they still will be able to see the spirochetes but there will be more little "dots" in the blood, and it's harder to see clearly.

If you took a treatment against cysts only, you could see many spirochetes.
And if you took a treatment against spirochetes only, you could see many cyst.

But don't worry, if you are infected and don't take the treatment, you will see spirochetes, if the guy has a technique to make the spirochetes exits the RBCs like someone already told you.

Regular Member

Date Joined Apr 2015
Total Posts : 77
   Posted 5/10/2018 5:27 PM (GMT -6)   
Thanks everyone for the advice! I had the exam last week. I did not remember to be completely fasted although it had been several hours. I did try stopping treatment 4 or 5 days ahead also.

The results were....no spirochetes noticed, but some probable of what he called "chronic Lyme" or similar - I think the cysts. A few other minor items also. It certainly wasn't chock full of baddies, but there were noticeable suspicious things. I was too travel-worn to ask anything about crushing, etc. RBCs.

Of course, afterward I poked around the web about it some more. Unsurprisingly, Wikipedia has some unflattering things to say about DFM for this kind of live-blood analysis... some of which may have some validity (like, I was surprised to see a box of generic glass slides sitting open the counter, with no cleaning or prep work done to them before applying my blood). OTOH at least one of the links I followed (to a Medicare paper) didn't seem to actually support the specific sentence it was cited for.

And, just because some people misuse or abuse a tool, doesn't mean it doesn't have some validity. And of course, as most of us here probably know by now, "absence of evidence is not evidence of absence".

Overall, I'm glad I went, although I wish it had been cheaper ($300 for my entire initial consult here, including the dfm). And this is now on my list for "things I'd try to get studied, improve and promote if I were a multimillionaire" smilewinkgrin .

Veteran Member

Date Joined Nov 2015
Total Posts : 4895
   Posted 5/10/2018 11:00 PM (GMT -6)   
u coulda bought your own used scope for that much...glad it was helpful

Veteran Member

Date Joined Oct 2015
Total Posts : 964
   Posted 5/11/2018 12:48 AM (GMT -6)   
Is there a place somewhere around Massachusetts to have this done? I remember I looked around a couple months ago, and couldn't find a place.
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