Band 41 is flagella of a spirochete - if it’s not lyme - then what ?
41 cross reacts with some viruses. On its own I'd say it doesn't mean very much. However, 31 is specific to Lyme so an IND result there definitely means something.
Band 41 can be Lyme, Relapsing Fever, Pintas and Yaws, Syphillis, and dental spirochetes.
If you don't have the above..... then coupled with the band 31 - along with symptoms...then you can hang your hat on lyme.
The testing is a backup to clinical diagnosis.