I have the double mutation of 677T. I spent about a month reading Amy Yasko’s work. It’s not as simple as just taking some B12 and methyfolate as there are other SNPs to consider. For example, I have a CBS (transulfation) pathway upregulation. If I take methyfolate, my methyl cycle begins operating, but since I have a transulfation uperegulation all the new homocysteine that I’m creating, instead of being re-methylated to keep the cycle going, gets sucked down the transulfation pathway and turned into sulfur ammonia and excess glutamate, all bad things. Imagine water circulating around a circular pipe, but there’s a leak. You get the pipe filled and water circulating, but half if it gets siphoned off down some side pathway.
In that case you need to supplement with yucca, molbydium and Sparga to get your sulfur levels down (which you can confirm w the pee sticks) and ammonia levels in check, and THEN supplement w sublingual b12 and methyfolate.
I literally read a couple hundred pages of biochemistry, learned the whole methyl cycle, went deep down the rabbit whole studying all my SNPs and polymorphisms...did everything I outlined above, and then some...
And the net result was...nothing. Didn’t notice a single thing, good or bad.
A couple of things to consider. You are working with the Eukaryotic methylation(in humans) but have not considered what is happening in Prokaryotes (bacteria). Their methylation is different if existent at all. I did what you did, learn the various biochemical pathways in humans to find and fix my problems. It helped to an extent but it wasn't until I considered what the bacteria were doing until I made real progress. We have more bacteria than human cells so it would make sense to deal with and treat the bacteria as much or more than the human cells. I mentioned something to Dr Yasko a few weeks ago about
something she said and she responded. After about
30 minutes, she amended her statement acknowledging what I said may be factual. I imagine she researched what I said and found the evidence that I had seen. I simple acknowledged that many disease states can be seen by one's bacterial profile. It is actually that profile which determines the disease state. When you see a gene like CBS699T, there are base pair changes that alter the gene when an epigenetic shift occurs. Those base pairs can come from excessive nucleotide formation in our GI tract, like say Proteobacteria forming high amounts of thymine and adenine or Actinetobacteria forming more guanine and cytosine. This can be reflected in our base pairing IMO and is in my gene profile. I say this to say if you have issues with transsulfuration and CBS, then alter the sulfur fixing bacteria in the GI tract, the desulfuromas. Eating less sulfur is one way to do this and this also reduces the methionine and cysteine in the human methylation pathway. We ultimately can't control all the bacteria in our GI tract, but we can control some of them. pH is the ultimate determiner of what bacteria live and what don't in our GI tract, but they are also affected by temperature, toxins, and what nutrients are available.