babesia has never really been high up the list for me.
its almost never discussed in Europe
i have never had any severe sweats or fevers or "air hunger" so there has been nothing to suggest it was responsible for my symptoms - at least nothing that wasn't just as well explained by Bart and or Lyme.
however - one of the little projects on my list was to try to do some home blood smears stained with giemsa stain and looking for anything untoward in the blood
I don't have the proper equipment - but i had enough to do the basics - and an old biological microscope that is functional if a bit dilapidated - so i thought i would at least do a first rough attempt and see what i could see.
there were a few issues - the stain was only relatively faint and so the contrast is lower than it could be - and the microscope lamp was not as adjustable as i would like - the only camera i have is an iphone strapped to the eyepiece so the photos are not great - but crisp images of red blood cells via light microscopy s challenging at the best of times
but what i found surprised me
i was aware that bart would likely be to small to see - unless in v high numbers - which is rare -
-mycoplasma can be more numerous and i have seen others have successfully stained it with giemsa -
but what i found looks to be too big for mycoplasma -
circular or spherical in shape and and only one occupant per cell -
and all of similar size - so very much like Babesia - esp babesia microtii -
but there are many many species of babesia and Theileria and more being discovered -
i dont think they are artefacts - as they are all inside the red blood cells - and all the same size
and they are picking up the stain and showing a lilac color - which is what pyroplasts are supposed to do
i will link to pictures of some found in dogs in Portugal that look v similar
my giemsa stained smear at approx 400x https://ibb.co/wrjrnpb
and another https://ibb.co/1lyk2bp
a paper about
a babesia microtii like organism in dogs in Portugal with a v similar image https://parasitesandvectors.biomedcentral.com/articles/10.1186/1756-3305-4-50/figures/1/
any microscopists here ?
any thoughts ?