I read the paper; do you work at a lab where you made the pills and did everything they did?
("Patients with >3 rCDI episodes were referred for FMT. rCDI was controlled with oral vancomycin. On the day of the procedure, related donors, previously screened for transmissible pathogens, provided ~100 grams of freshly passed feces. The fecal sample was suspended in 600-800 ml of prereduced PBS. Over 2.6 h, serial centrifugation at 400xg, 1000xg and 6000xg with interspersed decanting of the supernatant layer recovered pelleted fecal microbes in the sediment of the last centrifugation step. The sediment was resuspended in a minimal amount of PBS to allow micropipetting into #1 gelatin capsules (0.47 ml), and overcapsulated further with #0 and #00 capsules. The recipient stopped vancomycin on the day prior to the procedure and at 05:00 h on the procedure day underwent a colonic cleansing with picosalix. At 13:00 h, without antacid premedication, the recipient ingested freshly assembled capsules (n=24-34) over 5-15 minutes on an empty stomach. Prior testing of capsules showed that capsules remained intact for over 2 h at RT and ~1 h at 35o C in stirred liquid. qPCR testing of the sediment of donor and serial recipient fecal samples, prior to and a week, 1, 3 and 6 months post FMT, was performed")
In other words did you have access to centrifuges and resuspended the sediment in PBS into gelatin capsules and also #0 and #00 capsules? Did you do it on an empty stomach, do the colonic cleansing with picosalix and the donor was screened? Also wondering what you symptoms were precisely as in the extent of ulcers
Did you know the extent of inflammation visually or other or were you using symptoms as a measure or guide of how well this helped you. I'm very curious as to how long you've had UC, what other medications you're taking, if you stopped them before doing this - just as much information you could tell me please. Do you ever go into complete remission? What are your worst symptoms and the extent of your inflammation (bleeding, mucous, fever, anemia or more mild such as less pain and blood or no blood? Also how many bm's/day and what do they consist of prior to and after this treatment
It seems in making something like this at it would be safer to administer a home made version via enema and how were you certain you would not cause further infection from a problem donor?
Others who tried also respond please
Male: born '66, diag. hypoglycemic '70; epilepsy '82, UC '84, lost hearing one ear in '04, Remicade (1/10weeks), Imuran 50 mg/day, Apriso, oxycodone - pain; Xanax - simple partial seizures; Carbatrol X - grand mal seizures
Post Edited (Burli) : 2/27/2015 8:42:07 PM (GMT-7)